Boron 4. Iron: Iron is mainly absorbed by the plant in the ferrous form, but ferric ion may also be absorbed. It is estimated that approximately half of all proteins contain a metal. In plants, the photosynthetic electron transport between the two photosystems has to cover distances of a few hundred nanometers. Plastocyanin was found in a head-on orientation, as determined using pseudocontact shifts, similar to that in the Phormidium complex, in which the hydrophobic patch represents the main site of interaction on plastocyanin. Reviewed-Annotation score: -Experimental evidence at protein level i. c According to the Ramachandran plot. S/MARs contain sequences that strongly resemble the 21 bp DNA element found in S/MARs of the plastocyanin region. It takes part in electron transfer in photosystem I, in which the reversible conversion from Cu + to Cu 2+ + e-takes place. With these cultures the amount of PC which is sufficient for maximum photosynthesis in intact cells was determined to be about 1 to 1.5 PC/P700. Some cyanobacterial thylakoids also contain a caa3-type cytochrome c6 oxidase that requires three atoms of copper (1). The influence of the transcriptional enhancer of the pea plastocyanin gene ( PetE ) on the acetylation of histones was examined with chromatin immunoprecipitation (ChIP) experiments using antibodies that recognize acetylated or nonacetylated histones H3 and H4. The molar extinction coefficient (ε 597) of the copper chromophore was shown to be 4.5 x 10 3. Cloning and characterization of the plastocyanin region. The elements arsenic, copper and mercury have which of the following effect? (i,i ϩ 2) (i,i ϩ 3) (i,i ϩ 4) and (i,i ϩ 5). Many inorganic elements, because they are required for catalysis or as structural components in biomolecules, are essential nutrients for life. The plastocyanin contains some uncharacterized carbohydrate. Phaseolus vulgaris (Kidney bean) (French bean) Status. Treatment with Triton X-100 is most ef-fective. Measurements were made by … In a situation of poor nutrition, organisms economize on the use of these elements by choosing alternate chemistries to achieve the same function. Copper is activator of enzyme. Biological energy conversion relies on a well-defined sequence of redox reactions within the membrane-embedded electron transport chains of chloroplasts and mitochondria. Spinacia oleracea (Spinach) Status. Silicon. The copper content and molecular weight contrast with that of plastocyanin preparations from spinach (mol. However, the interaction in the cross-complex is dependent on electrostatics, similar to that in the Nostoc complex. Plastocyanin (Pc) is a copper-containing protein, which functions as an electron carrier between the cytochrome b(6)f and photosystem 1 (PS1) complexes in the photosynthetic electron transfer (ET) chain. The ET is mediated by His87 situated in the hydrophobic surface in the north region of Pc. The −259/−79 by promoter fragment is capable of conferring glucuronidase (GUS) gene expression on the minimal −90/+3 by 35S RNA promoter of CaMV and −51/+60 by plastocyanin promoter, regardless of its orientation. This model for the overall tumbling of plastocyanin provided a statistically significant improvement over the isotropic model, yielding an F statistic value of 4.7 for Cu(II) plastocyanin and of 5. In addition to the beta-sheets, the structure contains an alpha-helix from Pro47 to Lys54 that follows beta-strand 4. Copper 7. A large proportion of all proteins are part of this category. The -259/-79 bp promoter fragment is capable of conferring glucuronidase (GUS) gene expression on the minimal -90/+3 bp 35S RNA promoter of CaMV and -51/+60 bp plastocyanin promoter, regardless of its orientation. as a probe and the sequence of a 1.9-kb DNA fragment containing the plastocyanin gene was determined. Plastocyanin has 2 electron transfer paths. Between 2 and 5 nmoles plastocyanin per mg chlorophyll are extracted (see also Ref. Chlorine 8. PETE. Plastocyanin, a photosynthetic electron transfer catalyst, is the most abundant copper protein in these organisms. The following points highlight the top ten essential elements of Micronutrients. View Answer. An important question that can now be tackled is whether this organisation is related to the spatial and temporal expression pattern of the genes. With later Archean metabolisms, which cor-responds to one plastocyanin for 220 to 550.. 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