The literature review showed evidence of alternative handling methods sections, such as using tunnels or cupped hands when picking up mice and rats, having positive impacts on animal welfare and consequently possibly on data reliability, when compared to stressful traditional handling methods such as lifting by the tail. Gibbs Building, 215 Euston Road, London, NW1 2BE | Tel: 020 7611 2233 | Fax: 020 7611 2260 | [email protected], NC3Rs Skills and Knowledge Transfer award, Tunnel handling improves the welfare of mice undergoing repeated procedures. Mice can then be encouraged to climb into the beaker (Figures 31.3 and 31.4). Mice quickly habituate to tunnel handling and can subsequently be restrained by the scruff or tail base for procedures or health and welfare assessments without negating the positive impacts of the non-aversive capture. They can be placed back by allowing them to climb freely from the device into the cage (Figure 31.5). They were also less likely to defecate during handling and behavioural testing than their tail-handled counterparts. A study funded by the NC3Rs explored how handling methods influenced mice’s behavior during cognitive tasks. We evaluated a handling method using tunnels to tame laboratory mice (ICR) in the context of animal welfare and ease of handling. However, you can instead gently guide the mice into these clears handling tunnels. The researchers showed that mice of all strains and sex developed a consistent voluntary interaction in the ninth daily handling session among all the three handling methods – the tail, home cage tunnel, or "cupped" on open hand . During 1-week acclimation to handling and subsequent 1-week oral administration (once per day), voluntary interaction with the experimenter was much greater in mice handled by a tunnel compared to those picked up by the tail. A reel may also serve as means of transfer, as mice like to crawl into the dark tube or climb onto the device. These observations suggest that habituating mice to handling by either the cupping or tunnel method minimizes handling-related stress. Dr Henderson’s work complements findings by other researchers showing that single or repeated scruff restraint, single IP injection, repeated subcutaneous injection, repeated oral gavage and tattooing or ear tagging also do not negate the positive effects of non-aversive handling. Tips and strategies for rolling out the refined handling methods in your facility. Using a tunnel or cupped hand to pick up mice causes less anxiety than traditional tail handling. They observed that BALB/c mice found restraint more stressful than handling alone, and showed evidence of a stronger aversion to IP injections than isoflurane anaesthesia. These non-aversive methods also make handling easier for researchers and animal care staff, and enhance mouse performance in behavioural tests. Mice quickly habituate to tunnel handling and can subsequently be restrained by the scruff or tail base for procedures or health and welfare assessments without negating the … Experimental protocols often require mice to undergo repeated procedures that can cause pain or discomfort. Hear from champions based at facilities around the UK who have successfully implemented non-aversive mouse handling methods. Tunnel handling has been shown in several studies from around the world to reduce anxiety in mice. Using a computer mouse may not seem dangerous, but potentially wrist-driven actions repeated thousands and thousands of times have the potential to cause carpal tunnel syndrome. Request copies of our mouse handling poster for display in your facility. Read the underpinning research and related papers. Their work adds to the substantial evidence supporting tunnel handling as a way to improve the welfare of mice and reduce handling-related stress, even when experimental protocols require the use of more aversive procedures. These positive behavioural effects persisted even after repeated aversive procedures. The original research by Professor Hurst was funded by the BBSRC and the NC3Rs. This study shows that using a tunnel for routine handling reduces anxiety among mice compared to tail handling regardless of prior familiarity with tunnels. However, despite clear negative effects on mice's behaviour, tunnel … Need vole removal in your hometown? Professor Jane Hurst describes the evidence supporting refined handling techniques and practical tips for implementation. 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